![]() ![]() Lay SummaryĬell therapy has been proposed as an effective treatment method for bone regeneration using stem cells and osteogenic inducting mediators. ConclusionsĪccording to this finding, Symphytum could enhance osteogenic differentiation of MSCs and can be considered as a safe, low cost, and highly effective drug for bone regeneration. Also, quantitative analyses of ALP activity revealed that Symphytum 6 C caused the most increase in the ALP activity up to 4.04 ± 0.34 and 7.86 ± 1.38 on day 7 and 21, respectively. Symphytum 6 C had the most color intensity in ALZ and ALP staining assays. MTT assay results illustrated that Symphytum 200 C had a high cytotoxicity (to about 75.2 ± 3.12), so that lower potencies were used for the next experiments. The osteogenic induction ability of Symphytum has been assessed by alizarin red staining (ALZ) and quantitative alkaline phosphatase (ALP) activity assays and compared with the osteogenic standard medium. Cytotoxic effect of Symphytum on the MSCs was evaluated by MTT assay. MSCs were isolated from the rat’s bone morrow. In the present study, the ability of homeopathic remedy Symphytum officinale to induce osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rat BM-MSCs) was studied Methods Cell therapy has been proposed as an effective treatment method for bone regeneration using stem cells and osteogenic inducting mediators. ![]()
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